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Indiana University School of Medicine
Indiana University School of Medicine
Stark Neurosciences Research Institute

Microscopy Core

The microscopy core provides access to state-of-art microscope equipment for Stark Neurosciences Research Institute-affiliated members. Please reach out to request equipment demos or purchasing based on scientific need.

What can the Core do for you?

  • Help design and optimize experimental imaging and quantification.
  • Provide consultation for data analysis.
  • Train users on confocal microscopy.
  • Provide incubation stage for live imaging.
  • Provide access to data analysis software.
  • Regular courses to improve your imaging and analysis.

Core Services

  • Zeiss LSM900 is a laser-scanning confocal on a Zeiss Axio Observer 7 Inverted Microscope. The instrument includes an Airyscan2 module, which allows for super-resolution beyond the diffraction limit of traditional confocal microscopes. (~120nm XY, ~350nm Z).
  • Four exciter lasers: 405, 488, 561, and 640.
  • Four objectives
    • Plan-Neofluar 5x/0.16 WD=18.5mm
    • Plan-Apochromat 10x/0.45 WD=2.0mm
    • Plan-Apochromat 20x/0.8 WD=0.55mm
    • Plan-Apochromat 63x/1.40 WD=0.19mm OIL IMMERSION
  • Three fluorescence detectors, two traditional PMTs and one Airyscan detector for simultaneous imaging of up to three colors
  • Resolution adjustable up to 6144x6144 per frame
  • Adjustable bitdepth: 8-16bit
  • Okolabs stagetop incubation with 35mm dish and plate adapters

  • Nikon A1R is a laser-scanning confocal on a Nikon Ti-E inverted confocal microscope. The instrument includes a resonant scan mirror on the x-axis, allowing for extremely fast acquisition speeds compared to traditional galvo scan mirrors.
  • Four exciter lasers: 405, 488, 561, and 640nm
  • Four objectives:
    • 10x Plan Apo λ
    • 20x Plan Apo λ DIC
    • 40x Plan Fluor DIC – OIL IMMERSION
    • 60x Apo λ – OIL IMMERSION
  • Four detectors for simultaneous imaging of up to four colors. A spectral detector is available for unmixing of similar fluors or spectroscopy.
  • The resonant scanner is limited to 512 pixels on the x-axis, and can acquire a 512x512 image at up to 30 frames per second. Full galvo scanning at 512x512 can achieve 1-10FPS. Full galvo scanning can acquire at resolutions up to 4096x4096.
  • Bitdepth: 12 
  • No automated stage

  • Nikon Confocal Analysis Workstation with 3D deconvolution and General Analysis modules 
    • Elements AR 4.51
  • Experimental Design or Data Analysis Consultation

core leadership

Request Service

In need of services at the Microscopy Core?  Contact Riley Hannan for consultation and equipment access or request services below. 
70936-Hannan, Riley

Riley T. Hannan, PhD

Assistant Research Professor of Neurology

Core Co-Director

Read Bio

68202-Merchak, Andrea

Andrea R. Merchak, PhD

Assistant Research Professor of Neurology

Core Co-Director

Read Bio

headshot of Jeff Recchia-Rife

Jeffrey Recchia-Rife

Core Lab Manager

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a headshot of Tammy Fajfer

Tammy Fajfer

Research Centers/Cores Finance Manager

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