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Myeloproliferative Neoplasms (MPN) — FISH Analysis

CPT Code(s):  88237, 88275, 88271 (x per probe)

Service Code (IU Health): 53101259, 53100707, 53100640

Ordering Recommendation: FISH is useful for (1) detection of a neoplastic clone that is associated with chromosome abnormalities observed in patients with MPN, (2) to evaluate specimens in which standard cytogenetic analysis has been unsuccessful, and (3) to identify and track chromosome abnormalities for follow-up of patients and evaluate response to therapy.

Synonyms: FISH, hematopoietic malignancy, myeloproliferative neoplasms, MPN, chronic myelogenous leukemia, CML, polycythemia vera, PV, myelofibrosis, PMF, essential thrombocythemia, ET, fluorescence in situ hybridization

Methodology: Fluorescence in situ hybridization (FISH) analysis.

Performed: Monday through Friday

Reported: 2-3 days

Panels: MPN Panel - 5q, 7q, CEP 8/20q, BCR/ABL1/ASS Each probe may also be run individually. Also available: FIP1L1-LNX1-PDGFRA and PDGFRB

Specimen Requirements

Collect: Non-diluted bone marrow aspirate in green-top sodium heparin tube, heparinized syringe.

Specimen Volume: Preferred: 2 mL for normal WBC (decreased WBC requires more, and elevated WBC requires less); Minimum: 0.5 mL

Storage/Transport: Room temperature. Do not freeze or expose to extreme temperatures.

Unacceptable Conditions: Frozen specimens. Formalin-fixed specimens.

Stability: Ambient: 48 hours; Frozen: Unacceptable