| Assay | Xenium In Situ V1 | Xenium In Situ V2 |
|---|---|---|
| Overview | <500-gene panels |
5,000-gene panels |
| Chemistry | RNA v1 | Xenium Prime |
| Panels |
Pre-desinged panels
|
Human prime 5K Mouse prime 5K |
| Panel sizes | 50–480 genes |
5,000 genes |
| Panel customization |
Add on up to 100 genes to pre-designed panels Design fully custom panels up to 480 genes |
Add on up to 100 genes |
| Compatible species |
Human, mouse Additional species with custom panels |
Human, mouse |
| Compatible tissue types |
FFPE Fresh frozen TMA |
FFPE Fresh frozen TMA |
| Cell segmentation | DAPI, multimodal |
DAPI, multimodal |
| Same-section multiomics capabilities |
Gene expression Protein (IF) Morphology (H&E) Xenium Protein subpanels (human FFPE tissues) |
Gene expression Protein (IF) Morphology (H&E) |
| Throughput |
Xenium slide size: 10.45 mm x 22.45 mm Two Xenium slides per run About three days per run |
Xenium slide size: 10.45 mm x 22.45 mm Two Xenium slides per run Less than six days per run |
| Cost per run (two slides) |
$8,500-$8,700 for IU users $10,200-$10,440 for non-IU academic users $12,200-$12,700 for industry users |
$17,000 for IU users $20,400 for non-IU academic users $23,600 for industry users |