10x Genomics Visium Spatial Platforms

Assay	Visium HD	Visium HD 3'	Visium v2	Visium v1 3' gene expression
Overview	Protein coding gene coverage	Whole transcriptome coverage	Protein coding gene coverage	Whole transcriptome coverage
Chemistry	Probe-based	3' poly(A) capture-based	Probe-based	3' poly(A) capture-based
Resolution	2 μm x 2 μm barcoded squares single cell–scale resolution	2 μm x 2 μm barcoded squares single cell–scale resolution	55µm barcoded spot	55µm barcoded spot
Species	Human, mouse	Agnostic	Human, mouse	Agnostic
Compatible tissue types	FFPE Fresh frozen Fixed frozen	Fresh frozen tissue	FFPE Fresh frozen Fixed frozen	Fresh frozen tissue
Assay input	Tisses sectioned on glass slides	Tisses sectioned on glass slides	Tisses sectioned on glass slides	Visium slides
Compatible instruments	Visium CytAssist	Visium CytAssist	Visium CytAssist	Direct placement
Throughput options	Capture area size: 6.5 x 6.5 mm Large capture area 11 x 11 mm is coming soon Two capture areas per slide	Capture area size: 6.5 x 6.5 mm Two capture areas per slide	Capture area size: 6.5 x 6.5 mm 11 x 11 mm Two capture areas per slide	Capture area size: 6.5 x 6.5 mm Four capture areas per slide
Same-section multiomics capabilities	Probe-based gene expression Protein (IF) Morphology (H&E)	3' gene expression Morphology (H&E)	Probe-based gene expression Protein (immuno-oncology focused panel) Morphology	3' gene expression Protein Morphology
Cost per slide	$10,200-$11,000 for IU users $12,100-$13,000 for non-IU academia users $13,300-$14,500 for industry users	$11,000 for IU users $13,000 for non-IU academia users $15,000 for industry users	$6,500-$10,500 for IU users $8,000-$12,000 for non-IU academia users $9,500-$13,500 for industry users	$11,000 for IU users $13,000 for non-IU academia users $15,000 for industry users